Table 1. Primers used to generate the ΔfliK mutant*.
Primer # | Name | Direction | Sequence (5′–3′) |
1 | FliK KO F | Forward | agattacaagcaaaagtaac |
2 | FliK-Str-KO-Part 2-F | Reverse | ggtagtcggcaaataagaattttcttacgatgtagata |
3 | FliK-Strep-R | Forward | catcgtaagaaaattcttatttgccgactaccttggtg |
4 | FliK-Str-F | Reverse | tggagtagtgtgtatatgagggaagcggtgatcgccga |
5 | FliK-Str-KO-Part 1-R | Forward | caccgcttccctcatatacacactactccaatgaact |
6 | FliK KO R2 | Reverse | tagaacctactttcgaaagctaagcg |
*Underlined sequences indicate overlapping base pairs.