Skip to main content
. 2014 May 23;9(5):e98338. doi: 10.1371/journal.pone.0098338

Table 1. Primers used to generate the ΔfliK mutant*.

Primer # Name Direction Sequence (5′–3′)
1 FliK KO F Forward agattacaagcaaaagtaac
2 FliK-Str-KO-Part 2-F Reverse ggtagtcggcaaataagaattttcttacgatgtagata
3 FliK-Strep-R Forward catcgtaagaaaattcttatttgccgactaccttggtg
4 FliK-Str-F Reverse tggagtagtgtgtatatgagggaagcggtgatcgccga
5 FliK-Str-KO-Part 1-R Forward caccgcttccctcatatacacactactccaatgaact
6 FliK KO R2 Reverse tagaacctactttcgaaagctaagcg

*Underlined sequences indicate overlapping base pairs.