Table 5.
VGII subtyping SYBR MAMA Ct values and genotype assignments for VGIIa,b,c
| |
VGIIa_Assay_45211 |
VGIIb_Assay_502129 |
VGIIc_Assay_257655 |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Isolate ID | Strain type via MLST | VGIIa Ct Mean | non-VGIIa Ct Mean | Delta Ct | Type call via assay | VGIIb Ct Mean | non-VGIIb Ct Mean | Delta Ct | Type call via assay | VGIIc Ct Mean | non-VGIIc Ct Mean | Delta Ct | Type call via assay | Final Call |
| B6864 |
VGIIa |
17.2 |
30.5 |
13.3 |
VGIIa |
31.0 |
17.5 |
−13.5 |
non-VGIIb |
40.0 |
27.8 |
−12.2 |
non-VGIIc |
VGIIa |
| B7395 |
VGIIa |
19.8 |
33.5 |
13.7 |
VGIIa |
33.1 |
20.3 |
−12.9 |
non-VGIIb |
40.0 |
30.6 |
−9.4 |
non-VGIIc |
VGIIa |
| B7422 |
VGIIa |
18.3 |
33.6 |
15.4 |
VGIIa |
26.4 |
17.6 |
−8.8 |
non-VGIIb |
39.2 |
28.6 |
−10.6 |
non-VGIIc |
VGIIa |
| B7436 |
VGIIa |
18.6 |
31.7 |
13.1 |
VGIIa |
30.1 |
17.0 |
−13.2 |
non-VGIIb |
38.0 |
29.1 |
−8.9 |
non-VGIIc |
VGIIa |
| B7467 |
VGIIa |
20.5 |
37.3 |
16.8 |
VGIIa |
35.1 |
20.3 |
−14.7 |
non-VGIIb |
40.0 |
30.9 |
−9.1 |
non-VGIIc |
VGIIa |
| B8555 |
VGIIa |
17.1 |
31.2 |
14.1 |
VGIIa |
30.3 |
17.5 |
−12.8 |
non-VGIIb |
40.0 |
27.7 |
−12.3 |
non-VGIIc |
VGIIa |
| B8577 |
VGIIa |
20.8 |
36.8 |
16.0 |
VGIIa |
32.8 |
20.8 |
−12.1 |
non-VGIIb |
40.0 |
31.4 |
−8.6 |
non-VGIIc |
VGIIa |
| B8793 |
VGIIa |
15.1 |
29.8 |
14.7 |
VGIIa |
30.7 |
18.6 |
−12.1 |
non-VGIIb |
40.0 |
29.8 |
−10.2 |
non-VGIIc |
VGIIa |
| B8849 |
VGIIa |
19.8 |
34.4 |
14.6 |
VGIIa |
33.6 |
20.2 |
−13.4 |
non-VGIIb |
40.0 |
30.6 |
−9.4 |
non-VGIIc |
VGIIa |
| CA-1014 |
VGIIa |
13.1 |
27.3 |
14.2 |
VGIIa |
27.0 |
14.0 |
−13.0 |
non-VGIIb |
34.9 |
24.2 |
−10.7 |
non-VGIIc |
VGIIa |
| CBS-7750 |
VGIIa |
21.8 |
32.2 |
10.4 |
VGIIa |
33.4 |
21.5 |
−11.9 |
non-VGIIb |
40.0 |
34.1 |
−5.9 |
non-VGIIc |
VGIIa |
| ICB-107 |
VGIIa |
21.8 |
33.6 |
11.8 |
VGIIa |
33.2 |
21.2 |
−12.0 |
non-VGIIb |
40.0 |
33.8 |
−6.2 |
non-VGIIc |
VGIIa |
| NIH-444 |
VGIIa |
14.8 |
27.3 |
12.5 |
VGIIa |
28.5 |
15.3 |
−13.1 |
non-VGIIb |
36.1 |
25.7 |
−10.3 |
non-VGIIc |
VGIIa |
| B8508 |
VGIIa |
17.0 |
27.8 |
10.8 |
VGIIa |
26.5 |
17.3 |
−9.2 |
non-VGIIb |
31.7 |
22.7 |
−9.1 |
non-VGIIc |
VGIIa |
| B8512 |
VGIIa |
17.6 |
28.1 |
10.4 |
VGIIa |
26.3 |
18.0 |
−8.3 |
non-VGIIb |
33.2 |
24.2 |
−9.0 |
non-VGIIc |
VGIIa |
| B8558 |
VGIIa |
16.3 |
24.8 |
8.5 |
VGIIa |
27.3 |
15.3 |
−12.0 |
non-VGIIb |
29.4 |
20.0 |
−9.4 |
non-VGIIc |
VGIIa |
| B8561 |
VGIIa |
15.8 |
27.5 |
11.8 |
VGIIa |
25.0 |
16.9 |
−8.1 |
non-VGIIb |
33.4 |
23.2 |
−10.2 |
non-VGIIc |
VGIIa |
| B8563 |
VGIIa |
14.5 |
27.3 |
12.8 |
VGIIa |
23.9 |
15.6 |
−8.3 |
non-VGIIb |
31.7 |
21.7 |
−10.0 |
non-VGIIc |
VGIIa |
| B8567 |
VGIIa |
15.0 |
36.2 |
21.2 |
VGIIa |
24.5 |
16.0 |
−8.5 |
non-VGIIb |
31.8 |
22.2 |
−9.5 |
non-VGIIc |
VGIIa |
| B8854 |
VGIIa |
14.7 |
26.7 |
12.0 |
VGIIa |
24.1 |
15.1 |
−9.0 |
non-VGIIb |
31.4 |
22.2 |
−9.2 |
non-VGIIc |
VGIIa |
| B8889 |
VGIIa |
17.0 |
28.1 |
11.0 |
VGIIa |
25.9 |
17.3 |
−8.7 |
non-VGIIb |
33.2 |
23.8 |
−9.4 |
non-VGIIc |
VGIIa |
| B9077 |
VGIIa |
16.7 |
27.8 |
11.1 |
VGIIa |
25.6 |
16.7 |
−9.0 |
non-VGIIb |
32.9 |
24.4 |
−8.4 |
non-VGIIc |
VGIIa |
| B9296 |
VGIIa |
17.0 |
27.5 |
10.5 |
VGIIa |
25.5 |
17.3 |
−8.2 |
non-VGIIb |
32.9 |
24.8 |
−8.1 |
non-VGIIc |
VGIIa |
| B7394 |
VGIIb |
40.0 |
19.0 |
−21.0 |
non-VGIIa |
17.3 |
29.6 |
12.3 |
VGIIb |
40.0 |
29.0 |
−11.0 |
non-VGIIc |
VGIIb |
| B7735 |
VGIIb |
31.0 |
18.3 |
−12.8 |
non-VGIIa |
18.7 |
31.3 |
12.6 |
VGIIb |
38.1 |
28.9 |
−9.3 |
non-VGIIc |
VGIIb |
| B8554 |
VGIIb |
32.9 |
21.2 |
−11.7 |
non-VGIIa |
22.2 |
35.0 |
12.8 |
VGIIb |
40.0 |
30.4 |
−9.6 |
non-VGIIc |
VGIIb |
| B8828 |
VGIIb |
31.9 |
21.1 |
−10.8 |
non-VGIIa |
19.9 |
35.1 |
15.2 |
VGIIb |
40.0 |
30.5 |
−9.5 |
non-VGIIc |
VGIIb |
| B8211 |
VGIIb |
27.8 |
16.9 |
−10.9 |
non-VGIIa |
17.4 |
28.8 |
11.4 |
VGIIb |
32.3 |
22.3 |
−10.0 |
non-VGIIc |
VGIIb |
| B8966 |
VGIIb |
26.2 |
14.7 |
−11.5 |
non-VGIIa |
16.3 |
24.1 |
7.9 |
VGIIb |
31.8 |
23.2 |
−8.6 |
non-VGIIc |
VGIIb |
| B9076 |
VGIIb |
30.0 |
18.8 |
−11.2 |
non-VGIIa |
19.7 |
30.9 |
11.4 |
VGIIb |
39.1 |
27.0 |
−12.1 |
non-VGIIc |
VGIIb |
| B9157 |
VGIIb |
29.1 |
16.6 |
−12.4 |
non-VGIIa |
15.4 |
23.8 |
8.5 |
VGIIb |
30.3 |
21.3 |
−9.0 |
non-VGIIc |
VGIIb |
| B9170 |
VGIIb |
26.6 |
15.4 |
−11.2 |
non-VGIIa |
16.9 |
24.8 |
7.9 |
VGIIb |
31.0 |
22.7 |
−8.3 |
non-VGIIc |
VGIIb |
| B9234 |
VGIIb |
26.1 |
13.9 |
−12.2 |
non-VGIIa |
15.3 |
23.8 |
8.5 |
VGIIb |
30.2 |
21.2 |
−9.1 |
non-VGIIc |
VGIIb |
| B9290 |
VGIIb |
26.1 |
13.8 |
−12.3 |
non-VGIIa |
15.1 |
24.5 |
9.5 |
VGIIb |
30.6 |
21.2 |
−9.5 |
non-VGIIc |
VGIIb |
| B9241 |
VGIIb |
26.7 |
20.2 |
−6.5 |
non-VGIIa |
14.5 |
24.0 |
9.4 |
VGIIb |
30.5 |
21.4 |
−9.1 |
non-VGIIc |
VGIIb |
| B9428 |
VGIIb |
27.5 |
14.8 |
−12.6 |
non-VGIIa |
16.0 |
24.3 |
8.2 |
VGIIb |
32.0 |
22.4 |
−9.6 |
non-VGIIc |
VGIIb |
| B6863 |
VGIIc |
31.9 |
20.3 |
−11.5 |
non-VGIIa |
33.4 |
20.2 |
−13.2 |
non-VGIIb |
27.5 |
40.0 |
12.5 |
VGIIc |
VGIIc |
| B7390 |
VGIIc |
32.7 |
18.9 |
−13.8 |
non-VGIIa |
31.1 |
17.9 |
−13.2 |
non-VGIIb |
25.9 |
40.0 |
14.1 |
VGIIc |
VGIIc |
| B7432 |
VGIIc |
40.0 |
18.5 |
−21.5 |
non-VGIIa |
30.7 |
17.6 |
−13.1 |
non-VGIIb |
25.7 |
40.0 |
14.3 |
VGIIc |
VGIIc |
| B7434 |
VGIIc |
27.5 |
15.5 |
−12.0 |
non-VGIIa |
28.5 |
15.4 |
−13.1 |
non-VGIIb |
23.3 |
40.0 |
16.7 |
VGIIc |
VGIIc |
| B7466 |
VGIIc |
31.7 |
20.8 |
−10.9 |
non-VGIIa |
33.5 |
20.6 |
−12.8 |
non-VGIIb |
28.1 |
40.0 |
11.9 |
VGIIc |
VGIIc |
| B7491 |
VGIIc |
28.7 |
17.4 |
−11.2 |
non-VGIIa |
30.4 |
16.9 |
−13.5 |
non-VGIIb |
24.0 |
40.0 |
16.0 |
VGIIc |
VGIIc |
| B7493 |
VGIIc |
28.8 |
18.3 |
−10.6 |
non-VGIIa |
31.1 |
18.0 |
−13.1 |
non-VGIIb |
25.5 |
40.0 |
14.5 |
VGIIc |
VGIIc |
| B7641 |
VGIIc |
29.2 |
17.2 |
−12.0 |
non-VGIIa |
30.0 |
17.2 |
−12.8 |
non-VGIIb |
24.5 |
40.0 |
15.5 |
VGIIc |
VGIIc |
| B7737 |
VGIIc |
32.6 |
20.1 |
−12.5 |
non-VGIIa |
30.8 |
20.5 |
−10.4 |
non-VGIIb |
28.4 |
40.0 |
11.6 |
VGIIc |
VGIIc |
| B7765 |
VGIIc |
32.2 |
19.3 |
−12.8 |
non-VGIIa |
32.3 |
18.9 |
−13.3 |
non-VGIIb |
27.5 |
40.0 |
12.5 |
VGIIc |
VGIIc |
| B8210 |
VGIIc |
29.7 |
17.6 |
−12.0 |
non-VGIIa |
30.1 |
17.4 |
−12.7 |
non-VGIIb |
25.9 |
40.0 |
14.1 |
VGIIc |
VGIIc |
| B8214 |
VGIIc |
30.1 |
17.5 |
−12.5 |
non-VGIIa |
30.9 |
17.5 |
−13.4 |
non-VGIIb |
26.1 |
40.0 |
13.9 |
VGIIc |
VGIIc |
| B8510 |
VGIIc |
29.6 |
17.5 |
−12.0 |
non-VGIIa |
31.0 |
17.3 |
−13.7 |
non-VGIIb |
24.5 |
40.0 |
15.5 |
VGIIc |
VGIIc |
| B8549 |
VGIIc |
29.9 |
17.7 |
−12.1 |
non-VGIIa |
31.0 |
17.8 |
−13.2 |
non-VGIIb |
24.8 |
40.0 |
15.2 |
VGIIc |
VGIIc |
| B8552 |
VGIIc |
29.2 |
17.1 |
−12.0 |
non-VGIIa |
30.3 |
17.2 |
−13.1 |
non-VGIIb |
24.4 |
40.0 |
15.6 |
VGIIc |
VGIIc |
| B8571 |
VGIIc |
33.0 |
20.3 |
−12.7 |
non-VGIIa |
32.6 |
20.2 |
−12.5 |
non-VGIIb |
28.1 |
40.0 |
11.9 |
VGIIc |
VGIIc |
| B8788 |
VGIIc |
29.1 |
17.3 |
−11.7 |
non-VGIIa |
30.0 |
17.2 |
−12.8 |
non-VGIIb |
25.0 |
40.0 |
15.0 |
VGIIc |
VGIIc |
| B8798 |
VGIIc |
36.5 |
22.8 |
−13.7 |
non-VGIIa |
34.5 |
22.2 |
−12.3 |
non-VGIIb |
31.0 |
40.0 |
9.0 |
VGIIc |
VGIIc |
| B8821 |
VGIIc |
37.7 |
24.5 |
−13.2 |
non-VGIIa |
37.1 |
24.4 |
−12.7 |
non-VGIIb |
33.0 |
40.0 |
7.0 |
VGIIc |
VGIIc |
| B8825 |
VGIIc |
29.6 |
17.7 |
−11.9 |
non-VGIIa |
30.6 |
17.7 |
−12.9 |
non-VGIIb |
25.8 |
40.0 |
14.2 |
VGIIc |
VGIIc |
| B8833 |
VGIIc |
29.0 |
17.0 |
−12.0 |
non-VGIIa |
30.1 |
17.0 |
−13.1 |
non-VGIIb |
25.2 |
40.0 |
14.8 |
VGIIc |
VGIIc |
| B8838 |
VGIIc |
32.0 |
19.5 |
−12.5 |
non-VGIIa |
32.9 |
19.3 |
−13.7 |
non-VGIIb |
28.7 |
40.0 |
11.3 |
VGIIc |
VGIIc |
| B8843 |
VGIIc |
32.4 |
19.9 |
−12.5 |
non-VGIIa |
33.0 |
19.5 |
−13.5 |
non-VGIIb |
28.6 |
40.0 |
11.4 |
VGIIc |
VGIIc |
| B8853 |
VGIIc |
32.8 |
21.5 |
−11.3 |
non-VGIIa |
36.0 |
23.4 |
−12.6 |
non-VGIIb |
33.1 |
40.0 |
6.9 |
VGIIc |
VGIIc |
| B9159 |
VGIIc |
27.4 |
20.3 |
−7.1 |
non-VGIIa |
25.8 |
16.7 |
−9.1 |
non-VGIIb |
20.5 |
34.5 |
14.0 |
VGIIc |
VGIIc |
| B9227 |
VGIIc |
25.6 |
13.6 |
−12.0 |
non-VGIIa |
23.9 |
14.9 |
−9.0 |
non-VGIIb |
18.0 |
31.5 |
13.4 |
VGIIc |
VGIIc |
| B9235 |
VGIIc |
25.9 |
13.7 |
−12.1 |
non-VGIIa |
24.1 |
14.9 |
−9.2 |
non-VGIIb |
18.4 |
32.4 |
14.0 |
VGIIc |
VGIIc |
| B9244 |
VGIIc |
27.2 |
19.1 |
−8.1 |
non-VGIIa |
26.2 |
16.9 |
−9.2 |
non-VGIIb |
20.2 |
32.5 |
12.3 |
VGIIc |
VGIIc |
| B9245 |
VGIIc |
28.4 |
22.9 |
−5.5 |
non-VGIIa |
25.2 |
17.4 |
−7.8 |
non-VGIIb |
20.7 |
34.5 |
13.8 |
VGIIc |
VGIIc |
| B9295 |
VGIIc |
21.0 |
17.1 |
−3.8 |
non-VGIIa |
26.0 |
19.6 |
−6.4 |
non-VGIIb |
22.1 |
28.1 |
5.9 |
VGIIc |
VGIIc |
| B9302 |
VGIIc |
26.7 |
15.6 |
−11.1 |
non-VGIIa |
23.7 |
15.4 |
−8.3 |
non-VGIIb |
19.4 |
34.3 |
15.0 |
VGIIc |
VGIIc |
| B9374 | VGIIc | 27.4 | 21.6 | −5.8 | non-VGIIa | 24.0 | 15.3 | −8.7 | non-VGIIb | 19.4 | 33.4 | 14.0 | VGIIc | VGIIc |