Figure 2.

Cecropin A accumulates in seeds but not in vegetative tissues of rice plants. A. Immunoblot analysis of cecropin A in protein extracts (15 μg) from roots (R), leaves (L) and seeds (S) of wild-type (WT) and transgenic plants carrying the empty vector (EV) or the transgenes indicated. Lower panels show Ponceau staining of protein samples. B. In situ immunolocalization of cecropin A in WT and transgenic mature rice seeds. Representative images for each transformation event are shown (6 seeds per line, 3 lines per event and 2 independent assays). C. Cecropin A accumulation as estimated by immunoblot analysis of protein extracts from transgenic mature seeds in T3 generation compared to known amounts of synthetic cecropin A. The values correspond to the mean of estimated amounts of cecropin A per seed weight of at least three independent transgenic lines per transformation event and the bars to standard deviation. Results represent 3 independent experimental replicas.