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(a) The target cells (green) are BT-474 cells (breast carcinoma) labeled with EpCAM antibody conjugated with FITC fluorescence, and the background cells (red) are white blood cells labeled with CD45 antibody conjugated with Alexa Fluor 647 fluorescence. The target cells can be detected easily in all three ratios of mixtures. (b) The comparison of the detected data and calculated preset data with different ratios of breast cancer cells (BT-474) to white blood cells.
