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. 2014 May 20;11:96. doi: 10.1186/1743-422X-11-96

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Copyright © 2014 Mankotia and Irshad; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Amplification of N22 region of TTV genotype-1. N22 region was amplified using genotype specific primers. 1.2% agarose gel picture shows ~500 bp band corresponding to N22 region of genotype-1. Lane 1: 50 bp DNA marker; Lane 2 and 3: No template control; Lane 4–7: Bands of ~500 bp corresponding to N22 region amplified from TTV-DNA positive sera.