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. 2014 Mar 31;11:9. doi: 10.1186/1476-9255-11-9

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Copyright © 2014 Brittan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cytocentrifuge preparations of cells in BAL fluid following LPS inhalation. iPMLC (A), rPMLC (B), neutrophils (C), lymphocytes (D) and alveolar macrophages (AM) (E) were isolated from BAL fluid with high purity using FACS. PMLC are similar in morphology to blood monocytes and are a similar size to neutrophils, larger than lymphocytes and smaller than AM (Scale bar = 20 μm). Typical morphological features of each lineage were observed i.e. multi-lobular neutrophils, lymphocytes with high nucleus to cytoplasm ratio, and “fried egg”-shaped AM.