Table 1.
Primary antibodies used for flow cytometry and FACS
| Fluorochrome-conjugated antibody | Clone | Manufacturer, catalogue number | Laser filter, bandpass |
|---|---|---|---|
|
PMLC and Macrophages | |||
| Anti-human (h)CD14 PerCP/Cy5.5 |
HCD14 |
BioLegend, 325622 |
Blue, 695/40 |
| Anti-hCD16 PE/Texas Red |
3G8 |
Invitrogen, MHCD1617 |
Yellow/green (Y/G), 610/20 |
| Anti-hHLA-DR V450 |
G46-6 |
BD Horizon, 561359 |
Violet, 450/50 |
| Anti-hCD206 PE |
15-2 |
BioLegend, 321105 |
Y/G, 582/15 |
| Anti-hCD163 APC |
GHI/61 |
BioLegend, 333609 |
Red, 670/30 |
| Anti-hKi-67 PE |
Ki-67 |
BioLegend, 350504 |
Y/G, 582/15 |
| Anti-hCD71 FITC |
CY1G4 |
BioLegend, 334104 |
Blue, 530/30 |
| Anti-human mature macrophage marker (25 F9) APC |
eBio25 F9 |
eBioscience, 14-0115-82 |
Red, 670/30 |
|
Regulatory T cells | |||
| Anti-hCD3 FITC |
UCHT1 |
BD Pharmingen, 555332 |
Blue, 530/30 |
| Anti-hCD4 APC/Cy7 |
RPA-T4 |
BioLegend, 300518 |
Red, 780/60 |
| Anti-hCD25 PE |
BC96 |
BioLegend, 302606 |
Y/G, 582/15 |
| Anti-hCD127 PE/Cy5 | eBioRDR5 | eBioscience, 15-1278-42 | Y/G, 670/30 |
Staining panels were prepared using specific combinations of antibodies in order to analyse multiple cell subpopulations within a single sample. FITC = fluorescein isothiocyanate; PE = phycoerythrin; APC = allophycocyanin.