Figure 6.

RECQL5 Depletion Results in Transcription Stress across the Transcribed Region

(A–E) Mean AT frequency distribution of ±1 kb regions around RNAPII peaks in (A) wild-type, (B) RECQL5 knockdown cells (shRNA7), (C) exons, (D) introns, and (E) transcribed regions overall. See also Figure S3.

(F) CHIPMOD simulation of stochastic RNAPII pausing by 1% of the polymerase population. Parameters used, other than default: 9.99 kb gene; pause start at 5 kb; width 2.5 kb; 40% of normal (3.8 kb/min) elongation rate. Mean width of actual RNAPII ChIP-seq peaks is 2.7 kb; see Figure S4 for examples.