Figure 7.

RECQL5-Dependent Genome Instability Occurs in Areas of Elevated Transcription Stress

(A) Plot of distances from genomic loss-associated chromosomal breakpoints to the nearest RNAPII peak in the same gene. Left: breakpoints observed in the RECQL5 shut-off experiments. Right: simulated examples (100 independent trials, averaged by rank) of computer-generated breakpoints in genes across the human genome (as represented on the Nimblegen chip). Note that there will be fluctuation in the number of computer-generated distances due to the frequency of simulated breakpoints landing in genes with RNAPII peaks varying from simulation to simulation. Horizontal lines indicate median values (of the observed distances on the right, of the averaged randomized on the right).

(B) Plot of distances from chromosomal breakpoints (observed breakpoints on y axis and random computer-generated breakpoints on x axis) to their nearest RNAPII peak, regardless of either being in a gene.

(C) Model for the role of RECQL5 at the interface between transcription and the maintenance of genome stability. See text for details.