Figure S2.

RECQL5 Inhibits Transcription Elongation, Related to Figure 4

(A) Individual examples of DRB/GRO-seq traces before and after RECQL5 depletion, with computer-called wave fronts indicated (stippled lines; gray-shaded areas enlarged on right). Note that exons, presumably because of contamination with mRNA, often give a background signal (red asterisks above WT trace; refer to gene schematic below [exons as vertical bars]), which is taken into account by the wave front-calling protocol.

(B) Positions of RNAPII wave fronts calculated over appropriately long genes 25’ after DRB removal. No marked difference in the position of the wave fronts is observed in the RECQL5 KD cells compared to the control.