Figure 4.

NQO1 C609T SNP and NQO1 mRNA levels contribute to endogenous levels of NQO1 protein. Genomic DNA from a panel of OSCC cell lines was amplified using primers spanning the C609T SNP. PCR products were digested with HINf1α, and analysed by polyacrylamide gel electrophoresis to establish the presence of the SNP. (B) Total RNA was extracted from cells at approximately 80% confluency. RNA was reverse transcribed and the abundance of NQO1 mRNA was analysed relative to β actin mRNA using real time PCR. Histogram shows pooled results of 2- 3 independent experiments, analysed by one-way ANOVA, with Dunnet’s post-test relative to WHCO1.