Fig. 3.

The ring is a general feature of apoptosis. a γ-H2AX confocal immunofluorescence staining of Burkitt’s lymphoma Jurkat cells (clone A3) treated with Fas antibody (0.1 μg/mL). γ-H2AX is labeled in green; nuclei are stained in red with propidium iodide. The percentage of (apoptotic) cells with sub-G1 DNA is indicated to the right of each panel. b γ-H2AX confocal immunofluorescence staining of irradiated HCT116 cells (15 Gy). γ-H2AX is labeled in green; nuclei are stained in red with propidium iodide. Percentages of cells with sub-G1 DNA are indicated at right. c γ-H2AX confocal immunofluorescence staining in HCT116 cells treated with camptothecin (CPT; 25 μM, 23 h). γ-H2AX is labeled in red and P-S14-H2B in green; nuclei are stained in blue with DAPI. The percentage of cells with sub-G1 DNA is indicated at right. d γ-H2AX confocal immunofluorescence staining in HCT116 cells treated with cisplatin (CDDP; 50 μM, 23 h). γ-H2AX is labeled in red and P-S14-H2B in green; nuclei are stained in blue with DAPI. The percentage of cells with sub-G1 DNA is indicated at right