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Increased Smurf2 expression in aged mice. Quantitative RT–PCR analysis of Smurf2 expression in bone marrow (BM) and sorted LSK (Lin−Sca1+c-kit++) cells of young (2-month) and old (24-month) wild-type (+/+) and Smurf2T/T (T/T) mice. Relative expression in young wild-type cells was set to be 1 after normalization with β-actin. Error bars are SD of three independent experiments. Student’s t-test is used for statistical analysis. **P < 0.01, ***P < 0.001.
