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. 2014 Feb 4;13(3):478–486. doi: 10.1111/acel.12195

Figure 2.

Figure 2

Increased bone marrow (BM) cellularity and expanded long-term hematopoietic stem cell (LT-HSC) population in Smurf2-deficient mice. (A) Representative flow cytometry analysis of HSCs in BM of 2-month-old wild-type (+/+) and Smurf2T/T (T/T) mice. Live lineage-negative (Lin-) cells are gated and displayed for staining of Sca1 and c-Kit (left panels). LSK (Lin−Sca1+c-kit++) population is gated and displayed for staining of CD150 and Flt3 (right panels). LT-HSCs are defined as Lin−Sca1+c-kit++CD150+Flt3−, while Lin−Sca1+c-kit++CD150−Flt3− and Lin−Sca1+c-kit++CD150−Flt3+ are used to enumerate short-term HSCs (ST-HSCs) and multipotent progenitors (MPPs), respectively. (B) Average live BM cell numbers in young (2-month, N = 16) and old (24- to 30-month, N = 7) mice. (C) Frequencies of LT-HSC, ST-HSCs, MPP, and LSK cells as percent of live BM cells in young (2-month-old, N = 11) mice. (D) Average LT-HSC numbers in young (2-month, N = 11) and old (24- to 30-month, N = 6) mice. (E) Frequencies of LT-HSC, ST-HSCs, MPP, and LSK cells as percent of live BM cells in old (24- to 30-month, N = 6) mice. Error bars are SD and Student’s t-test is used for statistical analysis.