Figure 3.

Increased proliferation and decreased quiescence of Smurf2-deficient hematopoietic stem cells (HSCs). (A) Representative BrdU incorporation analysis of HSCs in wild-type (+/+) and Smurf2^T/T (T/T) mice. Long-term HSCs (Lin⁻Sca1⁺c-kit⁺⁺CD150⁺) are gated and displayed for BrdU staining. BM from mice without BrdU injection was stained for BrdU as a control. Average of three independent experiments is shown at right. (B) Representative cell cycle analysis of HSCs in wild-type (+/+) and Smurf2^T/T (T/T) mice. Long-term HSCs (Lin⁻Sca1⁺c-kit⁺⁺CD150⁺) are gated and displayed for staining of Ki-67 and DAPI. Average of three independent experiments is shown at right. Error bars are SD and Student’s t-test is used in statistical analysis.