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. 2014 Feb 4;13(3):478–486. doi: 10.1111/acel.12195

Figure 4.

Figure 4

Hematopoietic stem cells (HSCs) in aged Smurf2-deficient mice exhibit enhanced repopulating ability. Donor bone marrow (BM) from wild-type (+/+) or Smurf2T/T (T/T) mice (CD45.2+) mixed at 1:1 ratio with competitor BM of 2-month-old wild-type (CD45.1+/CD45.2+) mice are injected into lethally irradiated recipient mice (CD45.1+). Contribution of donors is determined by CD45 staining. Both young (2-month) and old (24-month) donors were used. (A) Donor (CD45.2+) contributions to B (B220+), T (CD3+), and myeloid (CD11b+) lineages in peripheral blood of recipient mice (N = 4) at indicated time post-BM transplantation. Two-way anova is used for statistical analysis. *P < 0.05, **P < 0.01, ****P < 0.0001, ns: not significant. (B) Donor contribution to BM and LSK (Lin−Sca1+c-kit++) cells in recipient mice (N = 3) at 6 months post-BM transplantation. Error bars are SD. Student’s t-test is used in statistical analysis.