Table 1.
Binding partners of KIBRA/kibra
| Name | Binding domains (or other influences on binding) | In vitro/In vivo | Ref. |
|---|---|---|---|
| Dendrin | WW domains of KIBRA | In vitro (Y2H) | (1) |
| PKCζ | The catalytic domain of PKCζ; C-terminus of KIBRA | In vitro (Y2H)/In vivo (co-localization) | (15) |
| Histone H3; DLC1 | Glutamic acid-rich region of KIBRA | In vitro (GST)/In vivo (IP) | (12) |
| SNX4 | Not determined | In vitro (Y2H)/In vivo (co-localization) | (20) |
| DDR1 | WW domains of KIBRA; the PPxY motif in DDR1 | In vivo (IP) | (10) |
| PATJ Synaptopodin |
The ADDV (PDZ) motif of KIBRA binds to the eighth PDZ domain of PATJ; WW domains of KIBRA bind to synaptopodin | In vitro (Y2H; GST)/In vivo (co-localization) | (9) |
| PKMζ | C-terminal region of KIBRA | In vivo (IP) | (9, 17) |
| Exocyst Sec 3 | Amino acids 129-525 of KIBRA | In vitro (Y2H)/In vivo (IP) | (19) |
| Lats1/2 | The N-terminal 86 amino acids of KIBRA and the PPxY motif in Lats2 | In vivo (IP) | (24) |
| Aurora-A | Ser 539 phosphorylation of KIBRA and amino acids 354-403 of Aurora-A. | In vivo (IP) | (29) |
| PP1 | The catalytic activity of PP1 is required for binding to KIBRA. | In vivo (IP) | (29) |
| CDC14A/B | CDK1 phosphorylation of KIBRAis involved | In vivo (IP) | (30) |
| RSK1/2 | Phosphorylation of KIBRA is required for interacting with RSK1, but not RSK2 | In vivo (IP) | (31) |
| FEZ1 | Coiled-coil domain of KIBRA | In vitro (GST; Y2H) | (34) |
| PAR3/PAR6β | Not determined | In vivo (IP; co-localization) | (14) |
| Citron (CIT) | PPxY motif of C-terminus of CIT kinase with WW domains of KIBRA | In vivo (IP) | (28) |
| AMOT | Not determined | In vivo (IP) | (28) |
| PICK1 | Not determined | In vitro (Y2H)/In vivo (IP; co-localization) | (5) |
| Merlin/(NF2); Expanded | The first WW domain of kibra and the ExRxPPxY motif | In vivo (IP) | (21–24, 27) |
| PTPN14 (Pez) | Not determined | In vitro (Y2H)/In vivo (IP) | (28,32) |
| Salvador (Sav) | WW domains of kibra and Sav | In vitro (Y2H)/In vivo (IP) (Drosophila) | (23) |
| Echinoid (Ed) | Intracellular domain of Ed | In vivo (IP) (Drosophila) | (26) |
Abbreviations: Y2H: yeast two hybrid; IP: immunoprecipitation; GST: Glutathione-S-Transferase pulldown