Table 1.

List of primers used for qPCR

Gene	Primer sequences (forward and reverse)
B2M	F: 5′-AAGTATACTCACGCCACCCA-3′
	R: 5′-CAG CGC TAT GTA TCA GTC TC-3′
IR	F: 5′-GTTCTTTCCTGCGTGCATTTCCCA-3′
	R: 5′-ATCAGGGTGGCCAGTGTGTCTTTA-3′
IRS-1	F: 5′- AGCCCAAAAGCCCAGGAGAATA-3′
	R: 5′-TTCCGAGCCAGTCTCTTCTCTA-3′
IRS-2	F: 5′-AGTAAACGGAGGTGGCTACA-3′
	R: 5′-AAGCTGAGAAGTCAAGGT-3′
PI3K	F: 5′-TAGCTGCATTGGAGCTCCTT-3′
	R: 5′-TACGAACTGTGGGAGCAGAT-3′
Akt2	F: 5-GAGGACCTTCCATGTAGACT-3′
	R: 5′-CTCAGATGTGGAAGAGTGAC-3′
GLUT4	F: 5′-ATTGGCATTCTGGTTGCCCA-3′
	R: 5′-GGTTCCGGATGATGTAGAGGTA-3′
PPARγ	F: 5′-GTCAGTACTGTCGGTTTCAG-3′
	R: 5′-CAGATCAGCAGACTCTGGGT-3′
Pgc1α	F: 5′-TACGCAGGTCGAACGAAACT-3′
	R: 5′-TGCTCTTGGTGGAAGCA-3′
TNFα	F: 5′-TAGCAAACCACCAAGTGGAG-3′
	R: 5′-AACCTGGGAGTAGACAAGGT-3′
IGF-1	F: 5′-CTGAGCTGGTGGATGCTCTT-3′
	R: 5′-CACTCATCCACACCTGT-3′