Figure 1. Domain architecture of human KNL1.

Key regions involved in KNL1 function are indicated schematically (top), and the corresponding amino acid sequences for each region in human KNL1 are listed (bottom). Aurora B kinase phosphorylates the N-terminus of KNL1 to inhibit association with PP1 (Liu et al. 2010). Mps1 phosphorylates the KNL1 MELT repeats to promote association with Bub1 and Bub3 (London et al. 2012; Primorac et al. 2013; Shepperd et al. 2012; Yamagishi et al. 2012). Amino acid residues 1-68 indicate the MT binding domain (Welburn et al. 2010), and underlined is a basic patch of residues (RRRH), whose analogous residues in C. elegans have been implicated in direct MT interaction (Espeut et al. 2012).