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Biofilm formation by E. coli DH5α, EAEC 042, tEPEC E2348/69, and the aEPEC strains LB10 and BA2468 after 1, and 18 days of incubation at 37°C. Bacteria were stained with propidium iodide (red). Prefixed HEp-2 cells stained with phalloidin-FITC (green). Panels (a) and (b) show a z profile through the structure. Panel (c): xz and yz sagittal images at selected positions in the biofilm are shown at the bottom and right side of images xy.
