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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 25;966:48–58. doi: 10.1016/j.jchromb.2013.11.043

Table 1.

Site-specific association equilibrium constants (Ka) for thyroxine and related compounds with HSA at 37°Ca

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Compound R3 R5 R3′ R5′ Chiral form Ka(M−1 × 105)a
Sudlow site I Sudlow site II
Thyroxine (T4) I I I I L-T4b
D-T4 		1.4 (± 0.1)
5.5 (± 0.9)		 5.7 (± 0.8)
29 (± 2)
Triidothyronine (T3) I I I H L-T3
D-T3 		0.170 (± 0.001)
1.45 (± 0.06)		 0.25 (± 0.02)
1.2 (± 0.2)
Reverse Triiodothyronine (rT3) I H I I L-rT3 1.99 (± 0.07) 3.3 (± 0.6)
Diiodothyronine (T2) I I H H L-T2 0.63 (± 0.03) 1.16 (± 0.06)
Thyronine (T0) H H H H L-T0 0.18 (± 0.02) -------
a

Each value in parentheses represents a range of ± 1 S.D. All association equilibrium constants were measured at 37ºC and pH 7.4. The data shown in this table was obtained from Refs. [53,54]. Sudlow site I and Sudlow site II are also known as the warfarin site and indole site, respectively.