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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Oncogene. 2013 Nov 25;33(48):5511–5522. doi: 10.1038/onc.2013.493

Figure 8. MMTV-Espl1 mice display early cell cycle defects and co-localization of BrdU positive (S phase) and PH3 (Ser28, mitotic phase) cells.

Figure 8

(A-B) Mid-pregnant mammary gland sections were stained for BrdU (red fluorescence) and Phospho Histone H3 (Serine 28, green fluorescence). Representative images are shown for MMTV-Espl1 and wild type (WT) mice (B) and quantified in (A) as a percentage of total cells positive for DAPI (p<0.0005). (C) Western blot shows increased Separase expression in MMTV-Espl1 mice mid-pregnant glands compared to WT. (n=3 animals used for each genotype, and ~200 cells per animal were analyzed).