Figure 2. Down-regulation of NPM1 expression in FLT3-mutated cells.
Panel A and B showed the results of down-regulation of NPM1 expression by EGCG treatment in IMS-M2 cells as well as MOLM13 control cells shown in Panel C. The cells at a density of 1×105 cells/ml were treated with 15, 30, or 60 µmol/L EGCG or DMSO alone (0 µmol/L EGCG) as control for 8 h (A and C) or treated with 60 µmol/L EGCG for an indicated duration (B). Total cell lysates were subjected to western blot analysis with indicated antibodies.