Figure 4.
Effect of NF-κB inhibitors on B16-hgp100 melanoma in DNA vaccine model. C57BL/6 mice were skin vaccinated by transfection with hgp100 DNA (2.5 mg/mouse) using a gene gun. NF-κB inhibitors were administered subcutaneously. Seven days later (Day 0), the vaccinated mice were challenged i.d. with 5 × 104 B16-gp100 cells. Tumor size (in diameter) (a) and the percent survival of test mice that developed tumors (b) were serially measured. (a: No vaccination, b: hgp100, c: hgp100 + 100 μg/mouse DsCE-I, d: hgp100 + 100 μg/mouse DsCE-I + PDTC, e: hgp100 + 100 μg/mouse DsCE-I + PDTC + SB203580, f: hgp100 + 1 μg/mouse LPS, g: hgp100 + 1 μg/mouse LPS + PDTC, h: hgp100 + 1 μg/mouse LPS + SB203580, i: hgp100 + 1 μg/mouse LPS + PDTC + SB203580)