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. 2014 Jun;24(6):954–962. doi: 10.1101/gr.170431.113

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© 2014 Roerink et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Pol theta mediates end joining of breaks in pol eta- and pol kappa-deficient animals. (A) Fecundity of single, double, and triple knockout mutants of pol theta and TLS polymerases pol eta and pol kappa. (B) Quantification and (C) representative pictures of RAD-51 immunostainings on germlines of the indicated genotype. Scale bar, 10 µm. (D) Schematic representation of the unc-22 reporter gene and spontaneous deletions (in red) isolated from either polh-1polk-1 or polh-1polk-1polq-1 mutant animals. Three of five deletions extended beyond the borders of the unc-22 locus.