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. 2014 Apr-Jun;15(2):62–70.

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Copyright © 2014 Avicenna Research Institute

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

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Calibration curve of human (panel A) and mouse (panel B) sperm fluorescence intensity at various extracellular pH. BCECF-loaded sperm were treated with 0.1% Triton-X 100 at various extracellular pH (pHe) and the fluorescence intensity was measured. Fluorescence intensity values were then plotted against pHe values to obtain the calibration curve.