Figure 6.

Extended DOF for fluorescence lifetime imaging of dorsal root ganglion neurons (DRGs). (A) Confocal imaging of DRG neurons expressing GFP. Images obtained at various focal depths, with a DOF of 2.1 μm. The image on the far right is the resulting z-projection of 7 images taken every 6 μm, between z = −18 μm and z = 18 μm (example images on the left). (B) Extended depth of field with 2-photon excitation and fluorescence lifetime imaging. The same sample was imaged with 2-photon excitation. Left, single frame obtained with a DOF of 20 μm. Center, average of 7 frames (same acquisition time as for each confocal image). Right, color-coded lifetime image of DRG neurons obtained from photons accumulated from 7 consecutive frames.