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Bicd1gt/gt motor neurons have increased cell surface binding of HCT. (A) Wild type and Bicd1gt/gt motor neurons were incubated on ice for 15 min with AlexaFluor555-conjugated HCT (grayscale) and fixed. Scale bar = 20 μm. A heat map representation of HCT staining has been added to better visualize the differences in binding between the two genotypes (right panels). (B) Quantification of the cell surface pool of HCT from three independent experiments (t-test, mean ± s.e.m., *p < 0.05).
