Table 1.
The results of 14 laboratories on 78 samples
| Laboratories | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | G | H | I | J | K | L | M | N | |
| RP-PCR results | ||||||||||||||
| GGGGCC-repeat expansion | ||||||||||||||
| Yes | 46 | 46 | 46 | 46 | 42 | 48 | 45 | 43 | 43 | 45 | 34 | 44 | 41 | 43 |
| No | 32 | 32 | 32 | 32 | 32 | 28 | 33 | 32 | 32 | 30 | 33 | 32 | 33 | 28 |
| Q | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 3 | 3 | 3 | 11 | 2 | 4 | 7 |
| False positive | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
| False negative | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
| Sensitivity (%) | 100 | 100 | 100 | 100 | 91.3 | 100 | 97.8 | 93.5 | 93.5 | 93.5 | 71.7 | 95.7 | 89.1 | 93.5 |
| Specificity (%) | 100 | 100 | 100 | 100 | 96.8 | 87.5 | 100 | 100 | 100 | 93.8 | 96.9 | 100 | 100 | 87.5 |
| Unclassified (%) | 0 | 0 | 0 | 0 | 5.1 | 2.6 | 0 | 3.8 | 3.8 | 3.8 | 14.1 | 2.6 | 5.1 | 9.0 |
| Amplicon-length analysis results | ||||||||||||||
| Numbers of amplicon | ||||||||||||||
| 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | N.D. | N.D. | N.D. |
| 1 | 54 | 54 | 55 | 56 | 56 | 54 | 54 | 51 | 56 | 54 | 51 | N.D. | N.D. | N.D. |
| 2 | 24 | 24 | 22 | 22 | 22 | 24 | 24 | 24 | 22 | 24 | 24 | N.D. | N.D. | N.D. |
| Q | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | N.D. | N.D. | N.D. |
| RP-PCR+amplicon-length analysis results | ||||||||||||||
| GGGGCC-repeat expansion | ||||||||||||||
| Yes | 46 | 46 | 46 | 46 | 46 | 48 | 45 | 43 | 43 | 43 | 34 | 44 | 41 | 43 |
| No | 32 | 32 | 32 | 32 | 32 | 29 | 33 | 32 | 32 | 30 | 34 | 32 | 33 | 28 |
| Q | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 5 | 10 | 2 | 4 | 7 |
| False positive | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| False negative | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
| Sensitivity (%) | 100 | 100 | 100 | 100 | 100 | 100 | 97.8 | 93.5 | 93.5 | 93.5 | 73.9 | 95.7 | 89.1 | 93.5 |
| Specificity (%) | 100 | 100 | 100 | 100 | 100 | 90.6 | 100 | 100 | 100 | 93.8 | 100 | 100 | 100 | 87.5 |
| Unclassified (%) | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 3.8 | 3.8 | 6.4 | 12.8 | 2.6 | 5.1 | 9.0 |
No, sample numbers without large GGGGCC-repeat expansion; N.D., not determined; Q, sample numbers of undecided or unclassified samples Yes: sample numbers with large GGGGCC-repeat expansion.