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Expression of virulence genes of parental (P) and L-form (L) L. monocytogenes ScottA-GFP 3 h post infection of P-388D1 macrophages determined by Real-Time qRT-PCR. The expression of hly, actA, dacA, lgt, iap, pgdA, and hpt is depicted as x-fold up- or down-regulation in L-forms, compared to parental Listeria (A). Detection of ActA by Western Blot (B) revealed its absence on L-forms.
