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. 2014 May 20;8:39. doi: 10.3389/fnana.2014.00039

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Copyright © 2014 Amrein, Becker, Engler, Huang, Müller, Slomianka and Oosthuizen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ki67+ proliferating cells (A,D) and DCX+ (B,E) or PSA-NCAM+ (C,F) young neurons in naked mole-rats (A–C) and highveld mole-rats (D–F). Note the staining of presumably myelinated fibers in (E arrow), which in some sections did not allow the identification of DCX+ cells in highveld mole-rats. Background in PSA-NCAM stained sections of naked mole-rats was considerably higher than in highveld mole-rats obscuring weakly stained cells. We therefore choose DCX for the quantitation of young neurons in naked mole-rats and PSA-NCAM for highveld mole-rats and Cape mole-rats (not shown). (A,E) are composites of two focal planes. Scalebars: 10 μm.