Figure 6.

Effects of XI-2, XI-A, and XI-I myosin tail domains on persistent ER cisternae and punctae identified with co-expressed GFP-HDEL. Left-hand panel is the first frame of a 50 frame movie. Middle two panels are cisternal persistency expressed as the sum of cisternae (morphometrically separated from tubules with a binary opening operation) that persist for at least five frames (7.9 s). The corrected cisternal persistency subtracts out regions of continuous flow (sum of five frame differences). The yellow-green-blue-black persistency scale represents persistency from 7.9 to 80 s. Highly persistent regions are those that are counted for Figure 7, cisternae being greater than 0.3 μm² and punctae less than 0.3 μm² and both persisting for at least a third of total movie time (black to green on the persistency scale with black representing persistency through the whole 80 s and green representing persistency for approximately 23 s). Note, control refers to cells only expressing the ER marker GFPHDEL (green, left hand panel).