Figure 2.

MSC2360844 and MSC2364588 block BCR- and TCR-mediated responses in lymphocytes and TLR-induced IFNα by pDC in human primary cells. (A) CD19⁺ B cells isolated from PBMCs of healthy donors were stimulated with anti-IgM and IL-4 for 4 days. Proliferation was measured by [³H]-thymidine incorporation. (B) CD19⁺ B cells isolated from PBMCs of healthy donors were stimulated with anti-Ig, CpG ODN 2006, and IFNα for 48 h. Cytokines in the supernatants were measured with CBA kits. (C) CD4⁺CD45RA⁻ T-cells isolated from PBMCs of healthy donors were stimulated with anti-CD3 and anti-CD28 for 5 days. Cytokines in the supernatants were measured with Luminex kit. (D) CD123⁺/CD303⁺ pDC cells isolated from PBMCs of healthy donors were stimulated with CpG ODN 2395 Type C for 18 h. IFNα in the supernatants were measured with ELISA kit. Difference between basal and stimulated level was used as 100%. Data shown are mean ± SEM and are representative of at least three independent experiments.