Table 1.
Proteome changes of endogenous HIV-1 Vpr induced C8166 cell death.
| Spot No. |
MASCOT Scores a |
Access No.b |
Protein Name |
Mw (kDa) |
pI c | Sequences of Peptides Identified d | Fold Changes e |
|---|---|---|---|---|---|---|---|
| 1 | 224 | P48616 | Vimentin | 54 | 5.06 | K.NLQEAEEWYKSK.F R.ISLPLPNFSSLNLR.E K.FADLSEAANRNNDALR.Q R.QVQSLTCEVDALKGTNESLER.Q K.TVETRDGQVINETSQHHDDLE.- R.LLQDSVDFSLADAINTEFKNTR.T |
−21.9 |
| 2 | 408 | P27348 | 14-3-3 protein | 28 | 4.68 | R.NLLSVAYK.N K.DSTLIMQLLR.D R.YLAEVACGDDR.K K.YLIANATNPESK.V R.SICTTVLELLDK.Y K.QTIDNSQGAYQEAFDISK.K R.KQTIDNSQGAYQEAFDISK.K K.TAFDEAIAELDTLNEDSYK.D |
+7.0 |
| 3 | 561 | P35232 | Prohibitin | 30 | 5.57 | R.NVPVITGSK.D K.EFTEAVEAK.Q R.FDAGELITQR.E K.DLQNVNITLR.I R.VLPSITTEILK.S R.IFTSIGEDYDER.V K.LEAAEDIAYQLSR.S R.KLEAAEDIAYQLSR.S R.NITYLPAGQSVLLQLPQ.- R.AATFGLILDDVSLTHLTFGK.E K.FGLALAVAGGVVNSALYNVDAGHR.A R.GVQDIVVGEGTHFLIPWVQKPIIFDCR.S |
−1.6 |
| 4 | 82 | P02787 | Serotransferrin [Fragment] | 79 | 6.81 | K.YLGEEYVK.A K.SASDLTWDNLK.G |
−2.8 |
| 5 | 107 | PO9211 | Glutathione S-transferase P | 24 | 5.43 | K.ASCLYGQLPK.F M.PPYTVVYFPVRGR.C K.ALPGQLKPFETLLSQNQGGK.T |
+4.3 |
| 6 | 94 | P52566 | Rho GDP-dissociation inhibitor 2 | 23 | 5.10 | K.TLLGDGPVVTDPK.A K.TLLGDGPVVTDPKAPNVVVTR.L |
+5.1 |
| 7 | 288 | Q99497 | Protein DJ-1 | 20 | 6.33 | K.VTVAGLAGK.D K.DGLILTSR.G R.DVVICPDASLEDAKK.E K.GAEEMETVIPVDVMR.R K.GLIAAICAGPTALLAHEIGFGSK.V K.EGPYDVVVLPGGNLGAQNLSESAAVK.E |
+5.2 |
| 8 | 293 | P15531 | Nucleoside diphosphate kinase A | 17 | 5.83 | R.GLVGEIIK.R R.GDFCIQVGR.N K.DRPFFAGLVK.Y K.FMQASEDLLK.E R.TFIAIKPDGVQR.G R.NIIHGSDSVESAEK.E R.VMLGETNPADSKPGTIR.G K.YMHSGPVVAMVWEGLNVVK.T |
+2.2 |
| 9 | 90 | O75347 | Tubulin-specific chaperone A | 13 | 5.25 | R.LVLDSVKLEA.- R.LEAAYLDLQR.I R.RLEAAYLDLQR.I |
+12.0 |
| 10 | 136 | Q01469 | Fatty acid-binding protein | 15 | 5.34 | K.ELGVGIALR.K K.MGAMAKPDCIITCDGK.N K.TTQFSCTLGEKFEETTADGR.K |
+2.3 |
| 11 | 215 | PO9382 | Galectin-1 | 15 | 5.34 | K.SFVLNLGK.D K.LPDGYEFK.F K.DSNNLCLHFNPR.F R.LNLEAINYMAADGDFK.I R.EAVFPFQPGSVAEVCITFDQANLTVK.L |
+45.6 |
| 12 | 245 | Q5REA8 | Thioredoxin domain-containing protein 17 | 14 | 5.40 | K.TIFAYFTGSK.D K.NLKVTAVPTLLK.Y K.TIFAYFTGSKDAGGK.S K.VTAVPTLLKYGTPQK.L K.SWCPDCVQAEPVVR.E R.YEEVSVSGFEEFHR.A |
+10.3 |
Note:
a
Only proteins with significant Mascot database matches (P<0.05) and scores (>68) are listed.
b
Swissprot database access number (http://ca.expasy.org/sprot/).
c
Theoretical isoelectric point calculated by Swissprot database at http://ca.expasy.org/sprot/.
d
Peptides identified by LC/MS/MS for the specific proteins.
e
Positive numbers show flod changes of up-regulation comparing the ad-vector infection group with ad-vpr infected group; negative numbers show down-regulation.