Table 1.
Target gene primer sequences and annealing temperature.
| Gene | Primer (5′ → 3′) | Annealing temperature (°C) |
|---|---|---|
| β-Actin | F CCTCATGCCATCCTGCGTCTG R TTGCTCGAAGTCTAGGGCAACATAG |
58 |
| TRF1 | F AAGTCCTGAAAGCCCTGAATC R TTCCTGTGCCTCCAAAATCT |
58 |
| TRF2 | F CTGTATTCATTTTGCTAACTTAG R ATTCTTAACACTCTCTAGAGTTG |
55 |
| TIN2 | F CTTCATTCCTACTAAACTACTTG R ACTGTAGAGACAGTTCTAGACCT |
57 |
| POT1 | F CAGAAAAGTGTGGATATG R AAGTAAAAGAAGTGTGGG |
55 |
| TPP1 | F GCGTGACGTCTCACATCG R ATCGTGATGGTTCTGCCTTC |
55 |
| RAP1 | F TGTGGTGTGTGTGTGTGTGG R CCCCACACACACCACACAC |
56 |