Figure 7.

Angpt2 induces proinflammatory chemokines and adhesion molecules in endothelial cells. (A) qPCR showing increased transcripts of CCL2, CX3CL1, ICAM1, and TGFB1 in FACS sorted aortic endothelial cells from mice with AdAngpt2. (B) qPCR for the transcripts of chemokines, adhesion molecules, and profibrotic cytokines in primary cultured aortic endothelial cells stimulated with recombinant Angpt2 (500 ng/ml) for 24 hours. Expression levels are normalized by glyceraldehyde 3-phosphate dehydrogenase. ^†P<0.01; ^‡P<0.001.