Figure 4.

Bioenergetic and redox impairment in flx1Δ strain: ATP and ROS content. Cellular lysates were prepared from WT and flx1Δ mutant strains grown in glycerol ((a), (b)) up to either the exponential (5 h) or the stationary phase (24 h) or in glucose ((a′), (b′)) up to the exponential phase (5 h). ATP content ((a), (a′)) was enzymatically determined following perchloric acid extraction and neutralization. ROS content ((b), (b′)) was fluorometrically measured as described in Section 2. The values reported in the histograms are the means (±SD) of three experiments performed with different cellular lysate preparations. Statistical evaluation was carried out according to Student's t-test (*P < 0.05).