Table 1.
Endogenous flavin content in spheroplasts and mitochondria.
| Carbon source | Strain | Spheroplasts | SCM | ||||
|---|---|---|---|---|---|---|---|
| FAD pmoli mg −1 | FMN pmoli·mg−1 | FAD/FMN | FAD pmoli mg −1 | FMN pmoli·mg−1 | FAD/FMN | ||
| Glycerol | WT | 157 ± 7 | 153 ± 7 | 1.1 | 160 ± 10° | 30 ± 10° | 4.8 |
| fl x1Δ | 126 ± 11 | 110 ± 10 | 1.1 | 140 ± 30° | 40 ± 10° | 4.5 | |
|
| |||||||
| Galactose | WT | 263 ± 10 | 189 ± 8 | 1.4 | 538 ± 32 | 103 ± 7 | 5.2 |
| fl x1Δ | 207 ± 8* | 195 ± 8 | 1.1 | 306 ± 15* | 67 ± 11* | 4.8 | |
Spheroplasts and mitochondria (SCM) were prepared from WT and flx1Δ cells grown in glycerol or galactose (2%) up to the exponential growth phase (5 h). FAD and FMN content was determined in neutralized perchloric acid extracts, as described in Materials and Methods. Riboflavin amount was not relevant, and thus its value has not been reported. The means (±SD) of the flavin endogenous content determined in three experiments performed with different preparations are reported. °Data published in (Bafunno et al., 2004) [26]; statistical evaluation was carried out according to Student's t-test (*P < 0.05).