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The antigenicity of rLigAni after adjuvant association. The immunoreactivity of rLigAni after association with either CpG or xanthan pruni adjuvants was determined by WB using monoclonal rabbit anti-LigA. Lane 1 has the Full-Range Rainbow Molecular Weight Marker (kDa); Lane 2 shows the purified rLigAni, which is used as a control; Lane 3 contains xanthan-PBS; Lane 4 has rLigAni-xanthan, and Lane 5 shows rLigAni-CpG-xanthan.
