Table 2.

SFRP2 and WIF-1 gene primer sequences for methylation-specific polymerase chain reaction assays

Gene	Primer (5’-3’)	Product size (bp)	Annealing temperature (°C)
M: SFRP2	GGGTCGGAGTTTTTCGGAGTTGCGC	138	62
	CCGCTCTCTTCGCTAAATACGACTCG		(38)
U: SFRP2	TTTTGGGTTGGAGTTTTTTGGAGTTGTGT	145	52
	AACCCACTCTCTTCACTAAATACAACTCA		(38)
M: WIF-1	GGGCGTTTTATTGGGCGTAT	197	58
	AAACCAACAATCAACGAAC		(38)
U: WIF-1	GGGTGTTTTATTGGGTGTAT	198	55
	AAACCAACAATCAACAAAAC		(38)

SFRP: Secreted frizzled related protein; WIF-1: Wnt inhibitory factor-1.