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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Immunol. 2014 Apr 25;192(11):5343–5353. doi: 10.4049/jimmunol.1400280

Figure 3.

Figure 3

PI3 kinase activation protects livers from IRI in myeloid PTEN KO mice. PTEN-loxP+/+ (WT) and Lyz-Cre+/− PTEN-loxP+/+ (KO) mice were used in our liver IR experiment as described in Material and Methods. A separate group of KO mice treated PI3K inhibitor, wortmannin (WM), prior to the start of liver ischemia was also included. (a) Liver IRI was evaluated at 6h post reperfusion by measuring sALT, liver histological analysis (H/E stain) scored by Suzuki Standard. Typical areas with edema (E), sinusoidal congestion (SC), cytoplasmic vacuolization (CV) and hepatocellular necrosis (N) were indicated. (b) Liver immune response against IR was determined by quantitative RT-PCR analysis of inflammatory gene expressions in sham-operated and IR livers. The ratios of target gene expression levels vs. housekeeping gene HPRT were plotted against different experimental groups. Representative results of 2 different experiments. N=3-4/group. *p<0.05, **p<0.01.