Fig. 2.
Activation of ERE reporter by ERα and its mutants in Huh-7 cells. (A) Nuclear extracts were prepared from Huh-7 cells that were transfected with expression plasmids for ERα and its A and D mutants and were subjected to Western blot analysis with an anti-ERα (αERαC) and an anti-TBP antibody. (B) A given expression plasmid was co-transfected with (ERE)3-luciferase reporter plasmid in Huh-7 cells and cells were treated with estradiol. (C) Co-transfected cells were treated with DES. (D) Co-transfected cells were treated with BPA. The results of luciferase reporter assays are reported as the mean of triplicate analysis with each experiment repeated three times.