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. Author manuscript; available in PMC: 2014 May 26.
Published in final edited form as: Steroids. 2012 Jan 15;77(5):448–453. doi: 10.1016/j.steroids.2012.01.001

Fig. 3.

Fig. 3

Activation of ERE reporter by ERβ and its mutants in Huh-7 cells. (A) Nuclear extracts were prepared from Huh-7 cells that were transfected with expression plasmids for YFP-tagged ERβ and its A and D mutants and were subjected to Western blot analysis with an anti-GFP antibody conjugated with HRP or an anti-TBP antibody. (B) A given expression plasmid was co-transfected with (ERE)3-luciferase reporter plasmid in Huh-7 cells and cells were treated with estradiol. (C) Co-transfected cells were treated with DES. (D) Co-transfected cells were treated with BPA. The results of luciferase reporter assays are reported as the mean of triplicate analysis with each experiment repeated three times.