Skip to main content
. Author manuscript; available in PMC: 2014 May 26.
Published in final edited form as: Steroids. 2012 Jan 15;77(5):448–453. doi: 10.1016/j.steroids.2012.01.001

Fig. 5.

Fig. 5

Mutation-specific increase of CCR7 and RAB27B mRNAs. Huh-7 cells were transfected with pcDNA3.1 ERα S212D, pcDNA3.1 ERα S212A or empty pcDNA3.1 for 24 h prior to treatment with 100 nM estradiol for an additional 24 h, from which total RNAs were prepared and subjected to real-time PCR and were subjected to real time PCR: CCR7 mRNA (A) and RAB27B mRNA (B). The mRNA levels are expressed relative to the levels of β-actin mRNA. Bars represent the mean ± SD. **significantly different at P < 0.01 (A) and * at P < 0.05 (B).