Table 5. Steady-State Kinetic Parameters of Sst2 and Its Substrate, Lys63-Linked Diubiquitin.
| sample | KM (μM) | kcat (s–1) | kcat/KM (×10–3 μM–1 s–1) |
|---|---|---|---|
| Sst2 | 18.8 ± 8.4 | 1.5 ± 0.2 | 79.8 |
| T319I | 19.7 ± 4.0 | 0.043 ± 0.002 | 2.2 |
| E286A | not determined | not determined | not determined |