Table 1. 16 Potentially Active Components in Fuzi Characterized by the Comparative NM/FM CMC Analysis System.

						[M + H]+m/z
peak no.	tR (1st NM/CMC, min)	RSD % of k values, n = 3 (NM/CMC)	tR (1st FM/CMC, min)	RSD % of k values, n = 3 (FM/CMC)	tR (2nd RPLC, min)	detected	expected	error (ppm)	formula	identification
1	2.5–15a	2.70	0.5–10a	2.98	0.91	424.2685	424.2694	–2.04	C23H37NO6	senbusine A
2	2.5–15	2.83	0.5–10	3.51	1.03	394.2596	394.2588	2.03	C22H35NO5	chuanfumine
3	7.5–15	1.57	7.5–20	0.99	1.35	330.2052	330.2064	–3.54	C20H27NO3	hetisine
4	5–17.5	2.22	2.5–15	5.98	2.05	408.2761	408.2744	4.04	C23H37NO5	isotalatizidine
5	5–17.5	3.21	2.5–7.5	3.96	2.2	358.2389	358.2377	3.43	C22H31NO3	songorine
6	5–15	1.21	5–15	2.57	2.45	454.2791	454.2799	–1.82	C24H39NO7	fuziline
7	2.5–10	4.47	2.5–7.5	8.19	2.68	438.2833	438.2850	–3.91	C24H39NO6	neoline
8	12.5–30b	0.96	10–30b	6.39	3.22	422.2911	422.2901	2.31	C24H39NO5	talatizamine
9	12.5–30b	1.61	10–30b	3.31	4.08	464.3024	464.3007	3.47	C26H41NO6	14-acetyltalatizamine
10	2.5–15	4.18	0.5–7.5	12.86	5.11	590.2936	590.2960	–4.02	C31H43NO10	benzoylmesaconine
11	2.5–12.5	3.46	0.5–7.5	20.10	5.50	604.3111	604.3116	–0.87	C32H45NO10	benzoylaconitine
12	7.5–20	2.93	2.5–12.5	1.49	5.54	558.3059	558.3061	–0.44	C31H43NO8	14-benzoyl-13-deoxyaconine
13	5–20	3.19	0.5–7.5	4.46	5.78	574.3036	574.3011	4.43	C31H43NO9	benzoylhypaconitine
14	5–20	4.41	2.5–12.5	4.26	6.20	588.3174	588.3167	1.18	C32H45NO9	benzoyldeoxyaconitine
15	10–25	2.31	7.5–25	6.81	6.51	542.3086	542.3112	–4.85	C31H43NO7	14-benzoylneoline
16	2.5–12.5	3.28	2.5–12.5	3.62	6.94	616.3126	616.3116	1.59	C33H43NO10	hypaconitine

^aRetention time of CMC demonstrated as time points of the start and the end of the elution curve.

^bPeak that was not completely flushed out by 1st-CMC column within 30 min.