Figure 1.

Running start assays for the human Y-family DNA polymerases on undamaged and damaged DNA templates. A preincubated solution containing 100 nM of 5′-[³²P]-radiolabeled (A, C, E, G, and I) 17-mer/40-mer or (B, D, F, H, and J) 17-mer/40-mer-8oxodG and either 100 nM of the indicated DNA polymerase or 25 nM of each Y-family polymerase was rapidly mixed with a solution containing all 4 dNTPs (200 μM each). The reaction mixtures were quenched at the indicated times with 0.37 M EDTA and resolved by using denaturing PAGE. The sizes of important products are indicated, and the 22nd position is denoted with an asterisk (*) to indicate an incorporation opposite the 8-oxodG lesion site. (K) The damaged 17-mer/40-mer-8oxodG substrate. The position of the 8-oxodG lesion within the template strand is indicated by a “Y.”