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Dose dependent inhibition of human colon cancer cell colony formation by M2 in HCT-116 (A) and HT-29 (B) cells. HCT-116 (A) and HT-29 (B) cells were treated with M2 (0, 1, 5, 10, 20, and 40 μM) and incubated in 6-well plates for 2 weeks, and the cells were then stained with crystal violet and counted for colony formation. Each column represents a mean ± SD (n = 3; *p < 0.05; **p < 0.01; ***p < 0.001).
