Skip to main content
. 2014 Apr 21;57(10):4111–4133. doi: 10.1021/jm500010b

Figure 4.

Figure 4

Putative binding mode of 21. (A) Surface of the Mcl-1 protein (PDB ID: 2NLA) is colored according to the chemical shift intensity. Significant shift (>0.09 ppm) is represented with purple, moderate shift (≥0.03 and ≤0.09 ppm) represented with pink. (B) Plot of chemical shift changes of Mcl-1 amide upon addition of 21 (Mcl-1:21 ratio of 1:2) as a function of Mcl-1 residue numbers. (C) Overlaid 15N–1H HSQC spectra of Mcl-1 (red) and in the presence of 21 (Mcl-1:21 ratio of 1:2) (black), (Mcl-1:21 ratio of 1:1) (purple). Arrows show the direction of chemical shift changes upon binding of 21. (D) Mcl-1 residues shown to be perturbed in HSQC NMR in the presence of 21 (Mcl-1:21 ratio of 1:2) (green).