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. 2014 May 19;7:228. doi: 10.1186/1756-3305-7-228

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Copyright © 2014 Gonçalves et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sensitivity of the LAMP assay. To determine the sensitivity of our assay we cloned the product of the LAMP reaction into a plasmid and then performed the assay using serial dilutions at the following concentrations: 10⁷ (1), 10⁵ (2), 10³ (3), 10¹ (4) and 10° (5). We performed the assay using both a thermocycler (A) and a heat block (B) with both proving suitable. Samples were incubated at 63°C for 90 min. The results were visualized on a 1.5% agarose gel stained with ethidium bromide. M = 1 Kb Plus, Invitrogen NC = negative control.